Lithium Isotope Fractionation in Weak Basic Solution Using Cation Exchange Chromatography

Separation of Monoclonal Antibodies by Weak Cation-Exchange Chromatography Using ProPac and ProSwift Columns

Monoclonal antibodies (MAbs) are currently developed by pharmaceutical and biotechnology companies for various therapeutic applications. MAbs undergo several post-translational modifications including oxidations, deamidations, lysine truncations, and glycan modifications. Manufacturing of MAbs and subsequent stability testing procedures involve routine analysis and monitoring of the impurities ...

Stable Sulfur Isotope Fractionation by Anion Exchange Chromatography. Production of Compounds Enriched in 34S

A separação do isótopo S foi obtida pela reação de troca isotópica en tre solução de ácido sulfuroso e ânions bissulfito adsorvidos em resinas aniônicas tipo amônio quaternário (Dowex 1X8 e Dowex 2X8, malha 100-200) acondicionadas em seis colunas de resina de troca aniônica. Cada coluna de resina possuia 130 cm de comprimento com diâmetro de 2,2 cm. As colunas foram conectadas em série du rante...

Immunoglobulin A Preparation from Human Pooled Plasma Using Plasma Fractionation and Ion Exchange Chromatography

Background: In this study we have prepared the IgA solution from normal human plasma using plasma fractionation and ion exchange chromatography. Materials and Methods: Using fractionation of plasma with cold ethanol (starting with 8% ethanol), fraction III was prepared as a suitable source for IgA preparation. Then it was treated with caprylic acid for separation of impurities. For enrichm...

Faster Separations Using Agilent Weak Cation Exchange Columns

The Effect of Temperature on Uranium Isotope Effects Studied by Cation Exchange Displacement Chromatography

The uranium isotope effect in the exchange system uranyl(VI)-malate ligand at 288–343 K has been studied by ion exchange displacement chromatography. At all temperatures 235U is enriched at the front of the uranium band. The single stage separation coefficient, (e =a – 1), increased from (0.9 ± 0.1)× 10 at 288 K to (2.9 ± 0.3)× 10–4 at 343 K. The equilibrium constant of the isotope exchange rea...